During this process, the viruses create long strings of double-stranded RNA ( dsRNA), which is not found in human or other animal cells. 在这一过程中,病毒创建长链的双链RNA,这一结构在人体和其他动物体细胞中没有发现。
In short, researchers in1998 discovered a class of double-stranded RNA ( dsRNA) that possessed powerful gene-silencing capabilities, or the ability to "turn off" disease-causing genes in the body. 简言之,研究者在1998年发现了一种极有利导致基因沉默的双链RNA,换言之它可以“关掉”身体内导致疾病的基因。
If a cell's defence mechanisms detect double-stranded RNA they destroy it, to protect against infection. 一旦侦测到,细胞的防御机制会消灭双链RNA,使细胞免遭感染。
Companies are trying to make drugs that consist of short sequences of double-stranded RNA, the chemical cousin of the DNA in genes. 这些公司试图制造由两个短的双RNA链组成的药,它是DNA基因的化学伙伴。
Double-stranded RNA does exist, but mostly in viruses. 双链RNA也存在,不过多数是在病毒中。
The small interference double-stranded RNA ( siRNA) can suppress gene expression with high efficiency and specificity. It has proven valuable in gene functionalization and gene therapy. 小分子双链RNA(siRNA)可以高效、特异地沉默目的基因表达,为基因功能研究及基因治疗提供了新工具。
Objective To construct the plasmid that can produce long, double-stranded RNA and identify nonspecific apoptosis induced by long, double-stranded RNA in human osteosarcoma cells. 目的通过构建能产生长双链RNA的质粒载体,探讨长双链RNA非特异性诱导人骨肉瘤细胞凋亡的可行性。
Direct Amplification of Target Sequences by Taq DNA Polymerise Using Double-stranded Rna as Templates 利用taqDNA聚合酶直接从双链RNA模板中扩增靶序列
RNA interference ( RNAi) is a newly described biological technology as genetic inhibitors of gene expression. RNAi is a process of sequence-specific posttranscriptional gene silencing mediated by double-stranded RNA. RNAi是双链RNA介导的转录后基因沉默的过程,是一种高效的高特异性抑制基因表达的新途径。
Sequence-specific Double-stranded RNA Releases CHIP Inhibition in TGF-β Signal Pathway 序列特异的双链RNA可解除CHIP对TGF-β信号通路的抑制作用
RNA interference ( RNAi) is a phenomenon in which the introduction of double-stranded RNA ( dsRNA) into certain organisms and cell types causes degradation of the homologous mRNA and it is a mechanism of post-transcriptional gene silencing. RNA干扰(RNAinterference,RNAi)是把双链RNA(double-strandedRNA,dsRNA)导入某些生物和细胞而引起同源mRNA降解的现象,是一种转录后基因沉默机制。
RNA interference is something of posttranscriptional gene silencing, specifically mediated by double-stranded RNA. RNA干扰是双链RNA特异诱导的转录后期基因沉默。
Virus Detection by Double-stranded RNA ( dsRNA) Analysis in Grape 葡萄病毒病的双链RNA(dsRNA)检测技术研究
Fire found that double-stranded RNA could very successfully suppress the expression of a homological gene in 1998, and this phenomenon was termed RNA interference ( RNAi). Fire等(1998)发现在线虫中双链RNA可以有效地阻断同源基因的表达,并将这一现象称为RNA干扰(RNAinterference,RNAi),从而获得了2006年的诺贝尔生理学/医学奖。
RNA interfere ( RNAi) is a process in which double-stranded RNA triggers the degradation of a homologous mRNA. RNA干扰(RNAi)是细胞内由双链RNA诱导降解与其配对的特定mRNA的过程。
RNA interference ( RNAi) is a phenomenon in which double-stranded RNA ( dsRNA) degrades its homological mRNA and leads to sequence specific post-transcriptional gene silencing ( PTGS) in organism. RNAi can generate gene knockdown by inhibiting the expression of protein. RNA干扰(RNAi)是指生物体内利用具有同源性的双链RNA(dsRNA)诱发序列特异的转录后基因沉默(PTGS)的现象,它可以通过抑制蛋白表达模拟基因敲除技术。
Construction of a Subtractive cDNA Library from the Paralichthys olivaceus Embryonic Cells Induced by a Double-Stranded RNA Virus 双链RNA病毒诱导的牙鲆胚胎细胞差减cDNA文库的构建
RNA interference ( RNAi) is induced by double-stranded RNA and silences the expression of homologous gene. RNA干扰是由双链RNA诱导的、关闭同源序列基因表达的机制。
Small interference RNA mixtures ( siRNA Cocktails) of Rabies virus ( RV) N gene, P gene and G gene were prepared by digesting long double-stranded RNA ( dsRNA) with RNase ⅲ. 采用RNaseⅢ消化长片段双链RNA的方法,制备了狂犬病毒N基因、P基因和G基因小干扰RNA库(siRNACocktail)。
The results showed that double-stranded RNA mediated gene silencing could be used as a functional tool to decrease the expression level of a specific gene in S. cerevisiae, which would contribute to the understanding of RNA interference in budding yeast. 结果表明,双链RNA介导的基因沉默技术,能够用作降低酿酒酵母某一特定基因表达水平的工具.并有助于理解芽殖酵母的RNA干扰现象。
RNA interference ( RNAi) is the process by which double-stranded RNA ( dsRNA) directs sequence-specific degradation of mRNA in animal and plant cells. RNA干扰是存在于动植物细胞中的,由双链RNA介导的,序列特异性的mRNA降解过程。
RNAi ( RNA interference) is a process of sequence-specific, post-transcriptional gene silencing in animals and plants initiated by double-stranded RNA that is homologous to the silenced gene and has proven as a novel approach to suppress gene expression specifically. RNA干涉(RNAinterference,RNAi)是指同源双链RNA所引发的特异序列的转录后基因沉默现象。它被证明是一项能有效而特异地抑制基因表达的新技术。
Usually, an inverted repeat sequence containing intron is taken into plants, transcripted in plants, formated a hairpin structure and produced double-stranded RNA, then caused gene silencing. 在植物中普遍采取的方法是通过转基因转入一段含有内含子的反向重复序列,通过转基因的方式转入植物体内并转录,形成发夹结构,产生双链RNA,从而引起基因的沉默。
Double-stranded RNA ( dsRNA) viruses have wide range of hosts. They have caused significant damage and economic losses to people or animals. dsRNA病毒宿主范围广泛,对人或动物造成了重大危害和经济损失。
RNAi is the sequence-specific gene silencing induced by double-stranded RNA ( dsRNA). 它是由双股RNA诱导的序列特异性基因沉默机制。
RNAi technology is a powerful experimental tool in laboratory. Using of homologous double-stranded RNA ( dsRNA)-induced sequence-specific silencing of target genes, it can rapidly block gene activity. RNA干涉(RNAi)技术在实验室中是一种强大的实验工具,它是利用具有同源性的双链RNA(dsRNA)诱导特异性的目标基因沉默,迅速降低基因表达水平。
RNA interference ( RNA interference, RNAi) is present in the eukaryotic organisms within a self-defense system to block the expression of exogenous genes, which is mediated by double-stranded RNA and enables the specific complementary single RNA degradation. RNA干扰(RNAinterference,RNAi)是存在于真核生物体内的一种阻断外源基因表达的自我防御体系。由双链RNA介导,能够特异性的使细胞内与其互补的单链RNA发生降解。
RNA interference ( RNAi) is a post-transcriptional gene silencing phenomenon induced by double-stranded RNA ( dsRNA), widely existing in fungi, plants and animals. RNA干扰(RNAinterference,RNAi)是一种由双链RNA诱发的转录后水平的基因沉默现象,广泛存在于真菌、植物和动物中。
It analysis showed that the target fragment sequence does not methylated, and methylation sites is media vector, therefore, the vector still in normal transcription of receptor cells play double-stranded RNA role. 3. 分析发现甲基化位点处于中间载体部分,目标片断序列未出现甲基化,因此,该载体仍能在受体细胞中起到正常转录双链RNA.的作用。